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FlyWire Proofreading Tips
In this document we’re going to go over some common problems you are likely to come across when proofreading in FlyWire. Using these tips you’ll learn how to better spot cell structure errors, and work around tricky spots in the dataset. X-Shaped Mergers These are what you’d traditionally think of as a merger, with a…
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How To Use FlyWire for Connectomics
Welcome to FlyWire, a community connectomics project that has mapped the entire adult Drosophila melanogaster (fruit fly) brain! For more information, see flywire.ai. Release 630 is the default view in FlyWire and contains reconstructions of the 127,978 neurons that were proofread in the Connectome publication <bioRxiv>. 72,403 (56%) of these cells have been typed or…
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Optic Lobe Tag Rally
The FlyWire connectome contains over 127,000 proofread cells, ~73,000 of which are in the optic lobes (OL). While ~50,000 cells in the brain have labels, as of this post (May 2023), only 27% of neurons in the optic lobes are labeled (~20K cells). We hereby request the help of all FlyWire community members to complete…
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One Year of Citizen Science in FlyWire
On April 28, 2022, the first citizen scientists were invited to FlyWire. Today, one year later the connectome nears completion and the flagship FlyWire publication is on the horizon. Our seventeen Flyers proofread 16,316 neurons and annotated 21,151 cells. Thank you for this truly phenomenal effort. The First annkri and bl4ckscor3 were the first Flyers…
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Hierarchical Cell Annotations in FlyWire
Guest post by Philipp Schlegel, Cambridge Connectomics/Jefferis Lab Dear FlyWire community, As promised during the town hall meeting on March 30, 2023, here is a neuroglancer link with a couple layers you might find useful: The annotation labels won’t work with the FlyWire neuroglancer (yet) but you should be able to drag & drop the other layers…
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Spring 2023 FlyWire Town Hall
You are cordially invited to the next FlyWire Town Hall this Thursday, March 30th at 11 am US ET. RSVP for Zoom link and to receive a reminder before the event starts at flywire.ai/townhall. The event will begin with remarks from Mala Murthy and Sebastian Seung. Sven Dorkenwald will next debut figures from the upcoming…
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How to find photoreceptors to proofread and label
Big thanks to FlyWire team member Szi-chieh for putting together this guide for finding photoreceptors! Introduction Drosophila has two chief motion detection pathways (reference): Since we have all Mi1 cells labeled in the left hemisphere, based on the motion detection pathways, we can work our way up using an individual Mi1 to locate its presynaptic…
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Make Paper Figures in FlyWire
FlyWire screenshots can be used in your Drosophila melanogaster publications. Here are some tips to easily set up your scene: Full screen 3D view Hover your cursor over the 3D part of the screen and hit SPACE. SHIFT+Click + Drag allows you to pan the camera. To remove the axis bars, use the keyboard shortcut:…
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Quest for the missing Photoreceptors (R cells)!
Calling all Flyers to a new quest! We’re on the hunt for unlabeled retinula cells, and we need your help to complete them! These photoreceptors extend into the retina, whereas our dataset terminates at the lamina. Therefore, it will not be possible to fully complete these cells, though we can observe large enough sections for…
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Cell Labeling/Adding Annotations
Proofreading cells is one of our main objectives in FlyWire. But as we near completion on the Fly connectome, we also need help labeling proofread cells, sometimes also referred to as cell annotation. This guide will get you started on your cell annotation journey, and provide resources to helpful tools that can assist in leveling…