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How to Use FlyWire
FlyWire is a community data resource containing whole brain connectome of the female adult fruit fly (FAFB dataset). As of October 2024, the flagship FlyWire paper, Neuronal wiring diagram of an adult brain, has been published in Nature, which includes 139,255 proofread neurons (Dorkenwald et al.) and over 100,000 cell label annotations. The FlyWire interface: After…
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Optic Lobe Tag Rally
The FlyWire connectome contains over 127,000 proofread cells, ~73,000 of which are in the optic lobes (OL). While ~50,000 cells in the brain have labels, as of this post (May 2023), only 27% of neurons in the optic lobes are labeled (~20K cells). We hereby request the help of all FlyWire community members to complete……
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How To Use FlyWire for Connectomics
Welcome to FlyWire, a community connectomics project that has mapped the entire adult Drosophila melanogaster (fruit fly) brain! For more information, see flywire.ai. Release 630 is the default view in FlyWire and contains reconstructions of the 127,978 neurons that were proofread in the Connectome publication <bioRxiv>. 72,403 (56%) of these cells have been typed or……
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FlyWire Winter ’23 Update
It’s officially the start of the fall and winter holidays here in the northern hemisphere. Time to break out the hot drinks and relax by the warmth of the computer while you do a little connectome exploration. It’s been a while since we updated the blog (and we’ll try to do this more frequently).ere is…
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Expert Proofreading Tips for the BANC (Brain and Nerve Cord) Dataset
Here are some proofreading tips specific to the BANC (Brain and Nerve Cord) dataset in FlyWire. Using Mitochondria For Continuations For this particular dataset, you may often find that the dark mitochondria in a branch cause the AI to grow confused and terminate the branch instead of finding the extension. However, although most mitochondria don’t…
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BANC Proofreading 101: Climb into Spelunker
Spelunker is the latest neuroglancer platform for proofreading and annotating large electron microscopy datasets. Whether you’re brand new to proofreading or or need some guidance while getting acquainted with the updated platform, this tutorial will help get going. Request access to BANC at https://flywire.ai/banc Interface overview Let’s begin by taking a look at the spelunker interface.…
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Visual Columns Mapping Challenge
If you are good at programming / data analysis / algorithms and like challenges read on! FlyWire is issuing its first Data Challenge. Data Challenge: A wiring diagram of a brain is modeled as a directed graph with weighted edges, where nodes represent neurons and edges represent synaptic connections (weight = # synapses). In this…
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BANC Guide for Citizen Scientists
Hello, intrepid explorers of the microscopic universe! FlyWire’s recent 4 year adventure into connectomics mapped the entire brain of a fruit fly (Drosophila melanogaster) at synapse resolution. We’re thrilled to announce the next endeavor: The BANC (Brain and Nerve Cord), a dataset generated by Jasper Phelps and Minsu Kim for Wei-Chung Lee’s Lab at Harvard…
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Banc-Bot Guide
Welcome to the BANC! We’re happy you’ve decided to join us to begin proofreading in the GNG (Gnathal Ganglia) of fruit fly brain and nerve cord! If you’re coming from Eyewire, or even if you are a seasoned Flyer, with this dataset we’ll be tracking progress a little differently. We’ll be using a little friend called…
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Finding Paths in Codex
With this tool you can specify one or more source cells + one or more target cells, set a minimum synapse threshold per connection, and get a matrix with shortest path lengths for all source/target pairs. From there, you can inspect / visualize the pathways between any pair of cells in detail.